A SILVER DIFFUSION METHOD FOR STAINING NERVE FIBERS IN PARAFFIN SECTIONS

Staining of Mycobacterium leprae by the Rio Hortega silver method in frozen and paraffin sections.

It has been recognized for some time that Mycobacterium leprae has the property of staining with the silver methods (2). However, there has not been described a simple, quick and easy technique for staining the bacillus in that way, applicable to either frozen or paraffin sections. It is my purpose to present a method which satisfies these requirements, and which also shows a new aspect of this...

Improved silver staining of nucleolar organiser regions in paraffin wax sections using an inverted incubation technique.

A new simple modification to the silver staining of nucleolar organiser regions (AgNORs) was devised which, by performing the incubation with the slide inverted, results in minimal undesirable background staining, a persistent problem. Inverted incubation is facilitated by the use of a commercially available plastic coverplate. This technique has several additional advantages over other publish...

Surface membrane staining of immunoglobulins in paraffin sections of non-Hodgkin's lymphomas using immunogold-silver staining technique.

The immunogold-silver staining (IGSS) method is a new immunostaining technique with much enhanced sensitivity for demonstration of antigens in paraffin sections. A series of 10 non-Hodgkin's lymphomas of B cell type were stained for surface membrane immunoglobulins by the IGSS and peroxidase-antiperoxidase (PAP) methods using paraffin sections and polyclonal primary antisera. The resulting stai...

Comparison of Three Staining Methods of Vibratome and Paraffin Sections in Preserving Thickness of Rat Hippocampal Sections

Purpose: To determine the optimal technique for maintaining hippocampus vibratome sections volume. Materials and Methods: This experimental study was performed on three rat hippocampus. The first hippocampus was embedded in paraffin and using rotary microtome forty sections were cut in a nominal thickness of 100 micrometer. They were then ordinary stained. The two others were cut into 80 sectio...

Silver Staining of Spirochaetes in Single Tissue Sections.